Evaluation of the Antifungal Efficacy of Acacia Niloticia, Ocimum Tenuiflorum incorporated into Short Term Soft Denture Liner: An In Vitro Study
Keywords:
Denture stomatitis, Candida albicans, Antifungal efficacy of Acacia, OcimumAbstract
Background and Objectives: Soft liner materials, though widely used in prosthodontics, have some physical and microbiological disadvantages. One such problem is the colonization of the denture surface by Candida albicans, thereby causing denture stomatitis. This study aims to evaluate and compare the inhibitory efficacy of Acacia, Ocimum, and Nystatin. Methodology: A total of 75 specimens were prepared by adding Ocimum, Acacia, or Nystatin to a commercially available soft liner in increasing order of concentrations. 5 ml of Sabouraud Dextrose broth (SDB) was poured into each test tube and autoclaved. The broth was inoculated with an entire loop of C. albicans for 24 hours at 37ºC. Discs were placed in the test tube and incubated for 24 hours at room temperature. The broth was removed with a sterile pipette after incubation. Discs were rinsed with sterile water to remove unattached C. albicans and sonicated in sterile water to remove surface organisms. The attached C. albicans were measured by inoculating on Sabouraud Dextrose agar (SDA). Results: Nystatin showed higher antifungal activity against C. albicans compared to Acacia nilotica and Ocimum tenuiflorum, and it was statistically significant. A. nilotica showed better antifungal activity than O. tenuiflorum, which was statistically significant. The higher concentration of these agents exhibited better antifungal activity, and the difference was statistically significant. Conclusion: Nystatin showed the best inhibitory effect on C. albicans, followed by A.nilotica and O. tenuiflorum. The Inhibitory effect increased statistically on increasing the concentration of these additives.
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